Coding

Part:BBa_J72027:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)


{Bmll46} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll46 {rbs_at_10000.VSV>}

Wobble mll46/mll47                (102, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll46         {rbs_at_10000.VSV>}
----
mll46    Forward wobble for {rbs_at_10000.VSV>} (Bmll46)
CCATAGAATTCATGAGATCTCAATAGAACATTAGATCAGAACGCGAAAGGGAACAATGTACACC
mll47    Reverse wobble for {rbs_at_10000.VSV>} (Bmll46)
CGTTAGGATCCCTTTCCCAGCCTGTTCATCTCTATATCGGTGTACATTGTTCCCTTTC


References